Jose Gimenez and Diego Godin netted in the last 12 minutes at the Wanda Metropolitano, to give themselves a chance of progressing.
With Juve having the Serie A title effectively wrapped up with 11 rounds remaining, they will channel all of their energy towards creating a comeback in this tie.
Kick off is 9pm West African time.
Juventus XI vs Atletico Madrid: Szczesny, Cancelo, Bonucci, Chiellini, Spinazzola, Can, Pjanic, Matuidi, Bernardeschi, Mandzukic, Ronald
Atletico Madrid XI vs Juventus: Oblak, Gimenez, Godin, Juanfran, Arias, Saul, Rodrigo, Lemar, Koke, Griezmann, Morata
Tuesday, March 12
21:00 Juventus vs Atletico Madrid